Clinical Research


17 years of clinical research

The patented formula in REJUVENATE is the result 40 years of medical research by Dr. Robert Wolfe and 24 human, clinical trials conducted at the University of Arkansas by Dr. Arny Ferrando.

This research led to the development of the perfect ratio of essential amino acids for optimal muscle recovery, maintenance and repair. For a nutritional supplement invention to be patented, the formulation or process that creates the food or supplement must be novel/unique from anything else on the market.

The patented essential amino acid technology in REJUVENATE is indeed unique from anything else in its category. After years of research on various test subjects; from rehabilitation patients, to cancer patients, to burn victims, the perfect ratio of essential amino acids were discovered.

What most, if not all, nutritional supplements on the market today currently lack is actual proof to back up their claims. REJUVENATE has real proof to back up its ground-breaking claims.

Studies from the Geriatric Center at Arkansas University Medical Center show a single serving of REJUVENATE starts to work within 30 mins, increasing the bodies ability to rebuild and repair of muscle by 57% - even at rest, with or without exercise.  Daily use of REJUVENATE will help slow and prevent muscle loss, with noticeable results in as little as 30 days. It’s the first muscle health supplement of its kind.

Source:
1. Transmembrane transport and intracellular kinetics of amino acids in human skeletal muscle.  Biolo G, RYD Fleming, SP Maggie, RR Wolfe Am. J. Physiol. 268 Endocrinol. Metab. 31:E75-E84, 1995.
2. Insulin Action on Muscle Protein Kinetics and Amino Acid Transport During Recovery After Resistance Exercise. Gianni Biolo, Bradley D. Williams, R.Y. Declan Fleming, and Robert R. Wolfe. Diabetes, Vol 48 May 1999. 
3. Exogenous Amino Acids Stimulate Net Muscle Protein Synthesis in the Elderly.  Elena Volpi, Arny A. Ferrando, Catherine W. Yeckel, Kevin D. Tipton, and Robert R. Wolfe. J Clin Invest, 101(9): 2000-2007, 1998.

4. Nonessential amino acids are not necessary to stimulate new muscle protein synthesis in healthy volunteers. K.D. Tipton, B.E. Gurkin, S. Matin, and R.R. Wolfe. J Nutr Biochem 10:89-95, 1999.

5. Oral amino acids stimulate muscle protein anabolism in the elderly despite higher first-pass splanchnic extraction. Volpi E, B Mittendorfer, SE Wolfe, RR Wolfe. Am J Physiology 277:E513-E520, 1999.

6. Basal Muscle Amino Acid Kinetics and Protein Synthesis in Healthy Young and Older Men. Elena Volpi, MD, Melinda Sheffield-Moore, Blake B. Rasmussen, and Robert R. Wolfe. JAMA September 12;286 (10): 1206-1212. 2001.

7. Timing of amino acid-carbohydrate ingestion alters anabolic response of muscle to resistance exercise. Tipton KD, BB Rasmussen, SL Miller, SE Wolf, SK Owens-Stovall, BE Petrini, RR Wolfe. Am J Physiol 281:E197-E206, 2001.

8. Essential Amino acids and muscle protein recovery from resistance exercise. Borsheim E, KD Tipton, SE Wolf, RR Wolfe. Am J Physiol. 283:E648-E657, Oct 2002. 

9. Acute response of net muscle protein balance reflects 24-h balance after exercise and amino acid ingestion. Tipton KD, E Borsheim, SE Wolf, AP Stanford, RR Wolfe. Am J Physiol 284:E76-E79, 2003.

10. Essential amino acids are primarily responsible for the amino acid stimulation of muscle protein anabolism in healthy elderly adults. Elena Volpi, Hisamine Kobayashi, Melinda Sheffield-Moore, Bettina Mittendorfer, and Robert R Wolfe. J Clin Endocrinol Metabo 89:4351-4358, Sept 2004. 

11. Independent and Combined Effects of Amino Acids and Glucose after Resistance Exercise. Sharon L. Miller, Kevin D Tipton, David L. Chinches, Steven E Wolf and Robert R. Wolfe. Medicine & Science in Sports & Exercise, 2002. 

12. Amino acid ingestion improves muscle protein synthesis in the young and elderly. Douglas Paddon-Jones, Melinda Sheffield-Moore, Xiao-Jun Zhang, Elena Volpi, Steven E. Wolf, Asle Aarsland, Arny A. Ferrando and Robert R. Wolf. Am J Physiol Endocrinol Metabo 286; E321-E328, 2004.

 13. Essential Amino Acid and Carbohydrate Supplementation Ameliorates Muscle Protein Loss in Humans during 28 Days Bedrest. Paddon-Jones D, M Sheffield-Moore, RJ Urban, AP Sanford, A Aarsland, RR Wolfe. J Clin Endocrinol Metabo 89:4351-4358, Sep 2004. 

14. Effect of an amino acid, protein and carbohydrate mixture on net muscle protein balance after resistance exercise. Bosheim E, Assland A and Wolfe RR. Int J Sport Nutrition Exercise Metabolism. Jun: 14 (3):255-71, 2004.

15. Exogenous amino acids stimulate human muscle anabolism without interfering with the response to mixed meal ingestion. Douglas Paddon-Jones, Melinda Sheffield-Moore Asle Aarsland, Robert R. Wolfe, and Arny A. Ferrando. Am J Physiol 288 (4): E761-767, Apr 2005. 

16. Aging is associated with diminished accretion off muscle proteins after the ingestion of a small bolus of essential amino acids. Christos S Katsanos, Hisamine Kobayashi, Melinda Sheffield-Moore, Asle Aarsland, and Robert R. Wolfe. Am J Clin Nutr 82:1065-73, 2005.

17. Differential stimulation of muscle protein synthesis in elderly humans following isocaloric ingestion of amino acids or whey protein. Douglas Paddon-Jones, Melinda Sheffield-Moore, Christos S. Katsanos, Xiao-Jun Zhang, Robert R. Wolfe. Exp Gerontol 41:215-219, 2006.

18. A high proportion of leucine is required for optimal stimulation of the rate of muscle protein synthesis by essential amino acids in the elderly. Christos S. Katsanos, Hisamine Kobayashi, Melinda Sheffield-Moore, Asle Aarsland, and Robert R. Wolfe. Physiol Endocriol Metab 291:E381-E387, Aug 2006. 

19. The deleterious effects of bed rest on human skeletal muscle fibres are exacerbated by hypercortisolemia and ameliorated by dietary supplementation. R.H. Fitta, J.G. Romatowski, J.R. Peters, D. Paddon-Jones, R.R. Wolfe, and A.A. Ferrando. Am J Physiol Cell Physiol 293:C313-C320, 2007.

20. Effect of Amino Acid Supplementation on Muscle Mass, Strength and Physical Function in Elderly Elisabet Borsheima, Quynh-Uyen T. Buib, Sandrine Tissierc, Hisamine Kobayashid, Arny. A. Clin Nutr 27(2):189-195, Apr 2008.

21. Amino acid supplementation decreases plasma and liver triglycerides in elderly. Elisabet Borsheim, Ph. D, Quynh-Uyen T. Bui, Sandrine Tissier, P.T., Melanie G. Cree, M.D., Ph.D., Ola Ronsen, M.D., Ph.D.,Beatrice-Morio, Ph.D., Arny A. Ferrando, Ph.D., Hisamine Kobayashi, D.V.M. Bradley R. Newcomer, Ph.D., and Robert R. Wolfe, Ph.D. Nutrition, 25(3):281-288, Mar, 2009.

22. Muscle Protein Synthesis and Balance Responsiveness to Essential Amino Acids Ingestion in the Presence of Elevated Plasma Free Fatty Acid Concentrations. Christos S. Katsanos, Asle Aarsland, Melanie G. Cree, and Robert R. Wolfe. J Clin Endocrinol Metab 94(8):2984-90, Aug 2009. 

23. EAA supplementation to increase nitrogen intake improves muscle function during bed rest in the elderly. Arny A. Ferrando, Doug Paddon-Jones, Nicholas P. Hays, Patrick Kortebein, Ola Ronsen, Rick H. Williams, Ashley McComb, T.Brock Symons, Robert R. Wolfe, William Evans. Clin Nutr 29:18-23, Feb 2010. 

24. Reginator demonstrates increase in muscle protein synthesis at low dosage trail of 3.6grams. Unpublished. 

25. Increased nitrogen intake following hip arthroplasty expedites muscle strength recovery. A.A. Ferrando, M.M. Bamman, S.E. Schutzler, H.J. Spencer, A.M. Dawson, R.P. Evans, R.R. Wolfe. Journal of Aging Research & Clinical Practice Volume 2, Number 4, 2013.